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US 2024/0191286 A1Published application
Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample
Assignee10x Genomics, Inc.
InventorsStephane Boutet, Katherine Pfeiffer
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Abstract
The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.
Claims30
A method for spatially tagging a nucleus of a cell of a biological sample, the method comprising:
- (a) contacting the biological sample with an array comprising a plurality of oligonucleotides, wherein an oligonucleotide of the plurality of oligonucleotides comprises:
- (i) a cleavage domain;
- (ii) a spatial barcode;
- and
- (iii) a sequence complementary to a capture domain;
- (b) cleaving the oligonucleotide from the array via the cleavage domain such that a portion of the oligonucleotide comprising the spatial barcode and the sequence complementary to the capture domain is introduced into the nucleus of the cell of the biological sample, thereby generating a plurality of spatially barcoded nuclei;
- (c) dissociating the biological sample into the plurality of spatially barcoded nuclei, wherein a spatially barcoded nucleus of the plurality of spatially barcoded nuclei is separated into a partition comprising a barcoded bead comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises:
- (i) a barcode and (ii) the capture domain;
- and
- (d) hybridizing the sequence complementary to the capture domain of the spatially barcoded nuclei to the capture domain of the capture probe, thereby spatially tagging the nucleus of the cell of the biological sample.
7 more elements
A method for spatially tagging a nucleus of a cell of a biological sample, the method comprising:
- (a) contacting the biological sample with an array comprising a plurality of oligonucleotides, wherein an oligonucleotide of the plurality of oligonucleotides comprises:
- (i) a cleavage domain;
- (ii) a spatial barcode;
- and
- (iii) a sequence complementary to a capture domain;
- (b) cleaving the oligonucleotide from the array via the cleavage domain such that a portion of the oligonucleotide comprising the spatial barcode and the sequence complementary to the capture domain is introduced into the nucleus of the cell of the biological sample, thereby generating a plurality of spatially barcoded nuclei and thereby spatially tagging the nucleus of the cell of the biological sample.
3 more elements
Dependencies are inferred from the claim text.