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US 2024/0191285 A1Published application
Compositions, methods and devices comprising stem-loop captor molecules
AssigneeGenecapture, Inc.
InventorsPaula M. KOELLE, Krishnan CHITTUR, Valentin KORMAN, Zachary McGEE
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Abstract
Disclosed herein are methods, devices and compositions comprising nucleic acid captor molecules with a stem region and a loop region for detecting target nucleic acids.
Claims27
A method for detecting target nucleic acid molecules, comprising,
- a) contacting target nucleic acids to captor molecules attached to a substrate of an assay device comprising,
- i) one or more types of captor molecules attached by a linker to the substrate, wherein individual captors are spaced apart from one another at a distance to prevent captor molecule-dimers;
- and
- ii) one or more general negative control captor molecules attached to the substrate;
- in buffering conditions that allow for hybridization of the target nucleic acids with captor molecules;
- b) adding a detectable probe that is capable of binding to a captor molecule;
- and
- c) detecting the amount, location on the substrate, or both, of the detectable probe.
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An assay device for detecting target nucleic acids, comprising
- a) a substrate
- b) one or more types of captor molecules attached to the substrate via a linker molecule and spaced apart from one another at a distance to prevent captor molecule-dimers;
- and
- c) one or more general negative control captor molecules attached to the substrate.
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A system for detecting target nucleic acids, comprising,
- a) an assay device for detecting target nucleic acids, comprising,
- i) a substrate;
- ii) one or more types of captor molecules attached to the substrate via a linker molecule and spaced apart from one another at a distance to prevent captor molecule-dimers;
- and
- iii) one or more general negative control captor molecules attached to the substrate;
- b) solutions comprising buffers or rinses;
- c) one or more detectable nucleic acid probes.
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Dependencies are inferred from the claim text.