Translocation control for sensing by a nanopore
AssigneeRoche Sequencing Solutions, Inc.
InventorsRobert M. McRuer, Mark Stamatios Kokoris
Life of this patent
Estimating the term…
Abstract
Translocation control for sensing by a nanopore, as well as methods and products related to the same, are provided. Such methods optimize duplex stability to provide high fill rate (of the hybridization sites) but do not prevent rapid dissociation required for high read rates, as well as controlling the translocation of a target molecule for sensing by a nanopore by use of a selective pulsed voltage. Products related to the same include a reporter construct comprising two or more phosphoramidites.
Claims20
A method for controlling the translocation of a target molecule for sensing by a nanopore, comprising passing the target molecule through the nanopore subjected to a base line voltage and a pulsed voltage, wherein the target molecule comprises two or more duplex features which provide translocation control by hybridization.
A reporter construct comprising a low impedance polymer and a high impedance polymer.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.