Tissue-specific methylation marker
AssigneeGrail, LLC
InventorsYuk-Ming Dennis LO, Rossa Wai Kwun CHIU, Kwan Chee CHAN, Wanxia GAI, Lu JI
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Abstract
Provided herein are compositions comprising tissue-specific markers for identifying a tissue of origin of a cell-free nucleic acid, e.g., a cell-free DNA molecule. Also provided herein are methods, compositions, and systems for identifying a tissue of origin of a cell-free nucleic acid by determining an absolute amount of cell-free nucleic acids comprising the tissue-specific marker. Also provided herein are methods, compositions, and systems for detecting a cancer in a tissue of an organism by analyzing tissue-specific markers.
Claims27
A method of measuring cytosine methylation at one or more differentiated methylated regions (DMRs), the method comprising:
- (a) obtaining cell-free DNA molecules from a first biological sample of a subject;
- and
- (b) performing an assay on the cell-free DNA molecules to measure, for each of the one or more DMRs, an amount of the cell-free DNA molecules comprising a first methylation status of a target sequence in the DMR;
- wherein (i) the one or more DMRs comprise a target sequence of one or both of Sestrin 3 (SESN3) and protein tyrosine kinase 2 beta (PTK2B);
- and (ii) the target sequence of each of the one or more DMRs comprises one or more CpG sites.
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Dependencies are inferred from the claim text.