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US 2024/0191279 A1Published application
Method and device for detection of myocardial infarction and reperfusion injury
AssigneeUniversity of Notre Dame du Lac
InventorsHsueh-Chia CHANG, Pinar ZORLUTUNA, Satyajyoti SENAPATI, Xiang REN
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Abstract
Described herein are methods and devices for rapid detection of miRNA and protein biomarkers for diagnosing myocardial infarction and reperfusion injury.
Claims32
A method for detecting microRNAs (miRNAs) associated with acute myocardial infarction reperfusion injury, or coronary artery disease, the method comprising:
- obtaining a biological sample from a subject;
- and
- performing an assay on the biological sample to detect one or more miRNAs selected from miR-1-1 (SEQ ID NO:
- 1);
- miR-208b (SEQ ID NO:
- 4);
- miR-499 (SEQ ID NO:
- 7);
- miR-200b (SEQ ID NO:
- 10);
- miR-543 (SEQ ID NO:
- 13);
- miR-331 (SEQ ID NO:
- 16);
- miR-3605 (SEQ ID NO:
- 19);
- miR-301a (SEQ ID NO:
- 22);
- miR-18a (SEQ ID NO:
- 25);
- miR-423 (SEQ ID NO:
- 28);
- miR-142 (SEQ ID NO:
- 31);
- or miR-132 (SEQ ID NO:
- 34) by hybridization with oligonucleotide probes complementary to the one or more miRNAs.
23 more elements
A method for differentiation between acute myocardial infarction reperfusion injury, or coronary artery disease, the method comprising:
- obtaining a biological sample from a subject;
- and
- performing an assay on the biological sample to detect one or more miRNAs selected from miR-1-1 (SEQ ID NO:
- 1);
- miR-208b (SEQ ID NO:
- 4);
- miR-499 (SEQ ID NO:
- 7);
- miR-200b (SEQ ID NO:
- 10);
- miR-543 (SEQ ID NO:
- 13);
- miR-331 (SEQ ID NO:
- 16);
- miR-3605 (SEQ ID NO:
- 19);
- miR-301a (SEQ ID NO:
- 22);
- miR-18a (SEQ ID NO:
- 25);
- miR-423 (SEQ ID NO:
- 28);
- miR-142 (SEQ ID NO:
- 31);
- or miR-132 (SEQ ID NO:
- 34) by hybridization with oligonucleotide probes complementary to the one or more miRNAs.
23 more elements
A method for detecting one or more cardiac-associated microRNAs (miRNAs), the method comprising:
- obtaining a biological sample from a subject;
- extracting from the biological sample a liquid component comprising exosomes and miRNA;
- subjecting the liquid component to an alternating current to lyse the exosomes and release exosomal miRNAs;
- concentrating the miRNAs proximate to an anion exchange membrane (AEM) functionalized with oligoprobes complementary to the miRNAs using a positively charged reservoir;
- generating a current-voltage curve (CVC) of the AEM and calculating the voltage shift from the CVC of the AEM absent miRNAs;
- quantifying the miRNAs bound to the oligoprobes using a calibration curve.
3 more elements
A system for simultaneous detection of multiple distinct microRNAs (miRNAs) in a biological sample from a subject, the system comprising:
- an integration board comprising:
- a piezoelectric substrate comprising:
- a first inlet, a first outlet, and a fluidic channel fluidly connecting the first inlet and the first outlet;
- interdigitated electrodes;
- and
- a transducer that applies alternating current to the interdigitated electrodes;
- a second inlet, a second outlet, and the fluidic channel fluidly connecting the first outlet, the second inlet, and the second outlet;
- one or more ion exchange arrays capable of detecting one or more distinct miRNAs, the one or more ion exchange arrays comprising:
- a first cation exchange membrane (CEM) positioned across the fluidic channel proximate to the second inlet;
- a second CEM positioned across the fluidic channel proximate to the second outlet;
- a positively charged reservoir fluidly connected to the fluidic channel by the first CEM;
- a negatively charged reservoir fluidly connected to the fluidic channel by the second CEM;
- an anion exchange membrane (AEM) functionalized with a plurality of oligoprobes specific for a plurality of distinct miRNAs, fluidly connected to the fluidic channel and positioned between the first CEM and the second CEM;
- at least 2 source electrodes adapted to apply current across the AEM;
- and
- at least 2 sense electrodes adapted to measure voltage across the AEM;
- a potentiostat;
- a binary coding and decoding circuit to control and modulate the detection sensing for each distinct miRNA;
- a plurality of probe selection switches;
- a power source;
- and
- a device for inducing fluid flow through the system.
19 more elements
A system for simultaneous detection of multiple distinct biomolecules in a biological sample from a subject, the system comprising:
- an integration board comprising:
- an inlet, an outlet, and a fluidic channel fluidly connecting the inlet and the outlet;
- one or more ion exchange arrays capable of detecting one or more distinct biomolecules, the one or more ion exchange arrays comprising:
- a first ion exchange membrane (IEM) positioned across the fluidic channel proximate to the inlet;
- a second IEM positioned across the fluidic channel proximate to the outlet;
- a first charged reservoir fluidly connected to the fluidic channel by the first IEM;
- a second charged reservoir fluidly connected to the fluidic channel by the second IEM;
- a third IEM functionalized with a plurality of probes specific for a plurality of distinct biomolecules, fluidly connected to the fluidic channel and positioned between the first IEM and the second IEM;
- a plurality of source electrodes adapted to apply current across the third IEM;
- and
- a plurality of sense electrodes adapted to measure voltage across the third IEM;
- a potentiostat;
- a binary coding and decoding circuit to control and modulate the detection sensing for each of the distinct biomolecules;
- a plurality of probe selection switches;
- a power source;
- and
- a device for inducing fluid flow through the system.
14 more elements
Dependencies are inferred from the claim text.