Tropane Alkaloid Transporters and Methods of Making Tropane Alkaloids Using the Same
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsChristina D. Smolke, Prashanth Srinivasan
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Abstract
Provided herein, among other things, is an engineered non-plant cell that produces a tropane alkaloid product, a precursor of a tropane alkaloid product, or a derivative of a tropane alkaloid product by means of a complement of biosynthetic enzymes and a complement of transporter proteins. A method for producing a tropane alkaloid, a precursor of a tropane alkaloid product, or a derivative of a tropane alkaloid product that makes use of the cell is also described.
Claims39
An engineered non-plant cell that produces a precursor of a tropane alkaloid product, a tropane alkaloid product, or a derivative of a tropane alkaloid product, wherein the engineered non-plant cell comprises a plurality of heterologous coding sequences encoding a plurality of enzymes within a pathway for producing the precursor of a tropane alkaloid product, the tropane alkaloid product, or the derivative of a tropane alkaloid product;
- wherein the cell comprises a plurality of heterologous coding sequences encoding a plurality of transporter proteins selected from the group of multidrug resistance transporter, pleiotropic drug resistance transporter, ATP-binding cassette transporter, multidrug and toxin extrusion transporter, nitrate transporter 1/peptide transporter family transporter, purine uptake permease-like transporter, and major facilitator superfamily transporter.
An engineered non-plant cell that produces a tropane alkaloid product or a derivative of a tropane alkaloid product, wherein the engineered non-plant cell comprises a plurality of heterologous coding sequences encoding a plurality of enzymes and a plurality of transporters within a pathway for producing the tropane alkaloid product or the derivative of a tropane alkaloid product.
Dependencies are inferred from the claim text.