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US 2024/0191253 A1Published application
Stable production systems for lentiviral vector production
AssigneeAsimov Inc.
InventorsJeremy J. Gam, Alec A.K. Nielsen
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Abstract
Described herein are lentiviral vector production systems. Also described herein are engineered cells and kits comprising a lentiviral vector production system and methods of using the same for lentiviral vector production.
Claims62
An engineered cell for lentiviral vector production comprising one or more stably integrated heterologous polynucleic acids collectively comprising:
- a nucleic acid sequence encoding for Gag;
- a nucleic acid sequence encoding for Pol;
- a nucleic acid sequence encoding for VSV-G;
- and a nucleic acid encoding for Rev;
- at least one of which is operably linked to a chemically inducible promoter.
2 more elements
A method of producing a lentiviral vector in an engineered cell, wherein the engineered cell comprises one or more stably integrated heterologous polynucleic acids collectively comprising:
- a nucleic acid sequence encoding for Gag;
- a nucleic acid sequence encoding for Pol;
- a nucleic acid sequence encoding for VSV-G;
- and a nucleic acid encoding for Rev;
- at least one of which is operably linked to an exogenous promoter that is capable of being bound by an exogenous transcriptional activator in the absence of a small molecule repressor, said method comprising:
- (a) introducing a transfer polynucleic acid into the engineered cell;
- and
- (b) introducing the exogenous transcriptional activator into the engineered cell;
- and
- (c) culturing the cell in the absence of the small molecule repressor, thereby inducing expression of Gag, Pol, VSV-G, and Rev;
- wherein (b) occurs before, concurrently with, or after (a).
8 more elements
Dependencies are inferred from the claim text.