Plant cell matrices and methods thereof
AssigneeCalyxt, Inc.
InventorsBenjamin Campbell, Lauren Harrison, Rachelle A. Lapham, Jonathan Mayers, Anders Uppgaard
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Abstract
Example embodiments in accordance with the present disclosure are directed to methods comprising contacting a plant part with a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation, and culturing the plant part under growth conditions to enhance PCM formation.
Claims54
A method comprising:
- contacting a plant part with a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation;
- and
- culturing the plant part under growth conditions to enhance PCM formation.
A method comprising:
- contacting a plant part with a bacterium strain containing a root-inducing (Ri) plasmid or a tumor-inducing (Ti) plasmid, a nucleotide sequence encoding a PCM-derived compound, and a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation;
- and
- culturing the plant part under infection and growth conditions to enhance transformation, induce the PCM formation, and induce production of the PCM-derived compound.
A method of generating a bacterium strain comprising:
- transforming a bacterium strain with a nucleotide sequence encoding the PCM-derived compound, wherein the bacterium strain comprises a nucleotide sequence encoding a gene that induces PCM formation or is transformed to comprise the nucleotide sequence encoding the gene that induces PCM formation;
- and
- culturing the transformed bacterium strain.
A plant cell matrix (PCM) culture comprising a plurality of core precursor compounds each produced at a level that is greater than production in tissue of a wild-type plant.
A method comprising:
- transforming a plurality of plant parts with a plurality of bacterium strains to induce plant cell matrix (PCM) formation;
- therefrom, assessing transformation frequencies of the plurality of bacterium strains;
- and
- selecting respective ones of the plurality of bacterium strains based on the transformation frequencies.
Dependencies are inferred from the claim text.