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US 2024/0191243 A1Published application
Hsv amplicon packaging system using engineered cells
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsJoseph C. Glorioso, III, Justus B. COHEN
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Abstract
Disclosed are custom HSV-1 packaging genomes for chromosomal integration into a cell line that is devoid of cis-acting HSV origins of replication (ori) and packaging sequences (pac) normally used for incorporation of the viral genome into virus particles, as well as the use of the resulting packaging cell for amplicon packaging.
Claims28
A packaging cell line comprising:
- (a) a helper virus genome;
- (b) a nucleic acid sequence encoding reverse tetracycline transactivator (rtTA);
- (c) a nucleic acid sequence encoding VP16;
- (d) a nucleic acid sequence encoding ICP0;
- (e) a nucleic acid sequence encoding ICP4;
- and
- (f) a nucleic acid sequence encoding ICP27.
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Dependencies are inferred from the claim text.