Regulation of gene expression through aptamer-modulated polyadenylation
AssigneeMeiragtx UK II Limited
InventorsMichael J. Volles, Olivier F. Danos, Xuecui Guo
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Abstract
The invention provides polynucleotide constructs for the regulation of gene expression by aptamer-based modulation of U1 snRNP-mediated suppression of polyadenylation and methods of using the constructs to regulate gene expression in response to the presence or absence of a ligand that binds the aptamer. The polynucleotide construct contains a U1 binding site in the context of a riboswitch comprising an effector region and an aptamer such that when the aptamer binds a ligand, target gene expression occurs.
Claims28
A polynucleotide cassette for the regulation of the expression of a target gene comprising a riboswitch wherein the riboswitch comprises an effector region and an aptamer, wherein the effector region comprises a U1 snRNP binding site and sequence complementary to the U1 snRNP binding site.
Dependencies are inferred from the claim text.