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US 2024/0191216 A1Published application
Recombinant host cells for the production of malonate
AssigneeLygos, Inc.
InventorsJeffrey A. Dietrich, Jeffrey L. Fortman, Eric J. Steen
Life of this patent
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Abstract
Systems and methods for the production of malonate in recombinant host cells.
Claims12
1-15. (canceled)
A malonyl-CoA hydrolase having at least 90%, 95%, or more sequence identity when aligned relative to SEQ ID NO:
- 53 and containing one or more of the following amino acid mutations at the aligned positions:
- S92C, H201N, R117D, R117E, R117N, R117Y, R117G, R117H, Q11D, QUE, Q11N, Q11Y, Q11G, Q111H, L93A, L93V, L93L, L93F, L93S, L93G.
A recombinant host microorganism that comprises a heterologous malonyl-CoA hydrolase selected from the group consisting of:
- a. SEQ ID No 53, wherein the serine residue at position 92 is mutated to a cysteine residue;
- b. SEQ ID No 53, wherein the arginine residue at position 117 is mutated to an aspartic acid, glutamic acid, asparagine, tyrosine, glycine, or histidine residue;
- c. SEQ ID No 53, wherein the glutamine residue at position 11 is mutated to an aspartic acid, glutamic acid, asparagine, tyrosine, glycine, or histidine residue;
- d. SEQ ID No 53, wherein the leucine residue at position 93 is mutated to an alanine, valine, isoleucine, phenylalanine, serine, or glycine residue;
- and
- e. SEQ ID No 53, wherein any combination of more than one residue listed in 2a-2d are mutated to the combinations of residues listed for each position.
3 more elements
Dependencies are inferred from the claim text.