Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
AssigneesWillow Biosciences, Inc., Epimeron USA, Inc.
InventorsTrish CHOUDHARY, Xueyang FENG, Gisele PASSAIA PRIETSCH
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to recombinant polypeptides that have prenyltransferase activity, nucleic acids encoding these recombinant polypeptides, recombinant host cells that produce these recombinant polypeptides, and compositions comprising the recombinant polypeptides, nucleic acids, and/or recombinant host cells. The present disclosure also relates to uses of these recombinant polypeptides, nucleic acids encoding them, and recombinant host cells comprising them, in methods for the preparation of cannabinoids.
Claims20
A recombinant polypeptide having prenyltransferase activity, wherein the polypeptide comprises an amino acid sequence of at least 80% identity to SEQ ID NO:
- 20 and an amino acid difference as compared to SEQ ID NO:
- 20 at one or more positions selected from:
- W61, F64, I79, F134, W153, F158, S175, S177, T180, N235, E284, and A293;
- optionally, wherein the amino acid differences are selected from:
- W61A, W61V, F64G, F64L, F64M, F64T, F64W, I179A, I79C, I79N, I79S, F134G, F134V, W153L, F158A, F158G, F158S, S175A, S175G, S175T, S175V, Y176S, S177A, S177G, S177T, T180I, T180L, T180R, T180V, N235C, N235K, N235V, E284D, E284K, E284R, A293G, A293K, and A293V.
Dependencies are inferred from the claim text.