Platforms and systems for automated cell culture

A cell culture container, comprising:

• a closed fluidic cell culture chamber comprising a first surface;
• a semi-transparent surface permanently attached to the first surface, wherein the semi-transparent surface is configured to:
• support a cell culture process performed on a cell culture adhered to the semi-transparent surface;
• and
• enable light-based cell imaging and light-based cell manipulation of the cell culture;
• one or more inlets for introducing fluid media into the cell culture chamber;
• one or more outlets for flushing fluid media and cells from the cell culture chamber;
• and
• one or more pluggable connectors configured to provide fluid and/or electrical communication between the cell culture container and a cell culture process module configured to manage the cell culture process.