Extracellular vesicles from mesenchymal stromal cells for treatment of diseases
OwnerKarl-Henrik GRINNEMO, Oscar SIMONSON +1inventors
InventorsKarl-Henrik GRINNEMO, Oscar SIMONSON, Sergey RODIN
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Abstract
The present invention relates to methods for obtaining extracellular vesicles (EVs) from cells such as mesenchymal stromal cells (MSCs), wherein the cells are cultured in the presence of polypeptides from the extracellular matrix proteins laminin alpha-5, laminin alpha-4 or their functional fragments, or in the presence of polypeptides comprising the extracellular domain of human MCAM protein. The invention further relates to EVs obtained by the above methods. The EVs are useful in the treatment and prophylaxis of medical conditions such as inflammatory diseases, ischemic heart disease and acute respiratory distress syndrome.
Claims21
A method for obtaining extracellular vesicles (EVs), comprising:
- (a) culturing multipotent stem cells, multipotent progenitor cells, or endothelial cells in a cell culture medium, in the presence of a composition comprising at least one polypeptide selected from the group consisting of:
- (i) a polypeptide comprising a human laminin α5 chain or a functional variant thereof;
- (ii) a polypeptide comprising a human laminin α4 chain or a functional variant thereof;
- and
- (iii) a polypeptide comprising the extracellular domain of human MCAM or a functional variant thereof;
- and
- (b) isolating extracellular vesicles from the cell culture medium.
17-22. (canceled)
Dependencies are inferred from the claim text.