Hydrogel Scaffolds And Uses Thereof
OwnerLay Poh TAN, Feng WEN +1inventors
InventorsLay Poh TAN, Feng WEN, Si Han Jasmine SEAH
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Abstract
The present invention relates to hydrogel scaffold and the uses thereof. In a specific embodiment, the hydrogel scaffold comprises gelatin methacryloyl (GelMA), alginate and pectin, and one or more cell types. In another embodiment, the one or more cell types comprise stem cells and/or progenitor cells. The stem cells are adipose-derived stem cells (ADSCs) or mesenchymal stem cells. The present invention further relates to the method of fabricating the hydrogel scaffold as well as a method of myogenic differentiation with the hydrogel scaffold.
Claims17
A hydrogel scaffold comprising:
- about 3.0-10.0 w/v % gelatin methacryloyl (GelMA);
- about 0.5-2.0 w/v % alginate;
- about 0.5-2.0 w/v % pectin;
- and
- one or more cell types.
A method of fabricating a hydrogel scaffold, the method comprising:
- a) preparing a solution comprising alginate and pectin;
- b) treating the solution from step a) to a temperature of about 4-120° C.;
- c) adding gelatin methacryloyl (GelMA) to the heat-treated solution from step b);
- d) adding one or more cell types to solution from step c);
- e) setting an injection rate of about 0.1-2.0 ml/min;
- f) wet-spinning a hydrogel scaffold.
Dependencies are inferred from the claim text.