Media and methods for growing mammary organoids
AssigneeStemcell Technologies Canada Inc.
InventorsDavid ROWBOTHAM, John STINGL
Life of this patent
Estimating the term…
Abstract
Disclosed are media, kits and methods for directed formation of mammary organoids. Embodiments of organoid media may be used to bias/enrich the formation of luminal organoids from isolated mammary epithelial cells. Embodiments of modified organoid media may be used to bias/enrich the formation of mixed lineage organoids from isolated mammary epithelial cells, or to subvert luminal organoids to mixed lineage organoids.
Claims29
A method of forming mammary organoids from isolated mammary epithelial cells, comprising:
- a) contacting the mammary epithelial cells with an organoid medium free of one or both of an exogenously-added WNT signaling agonist and/or an inhibitor of BMP signaling;
- and
- b) culturing the mammary epithelial cells in the organoid medium for a time sufficient to form a first population of organoids enriched for organoids composed of more luminal cells than non-luminal cells,
- wherein the organoid medium includes a basal medium and one or both of a ligand of ERBB1 and/or a ligand of ERBB4.
3-6. (canceled)
(canceled)
(canceled)
16-17. (canceled)
19-20. (canceled)
A mammary organoid medium, comprising:
- a basal medium and one or both of a ligand of ERBB1 and a ligand of ERBB4;
- and lacking one or both of an exogenously-added WNT signaling agonist and an inhibitor of BMP signaling.
(canceled)
(canceled)
(canceled)
(canceled)
34-38. (canceled)
Dependencies are inferred from the claim text.