Method for producing pluripotent stem cell population
AssigneeKaneka Corporation
InventorsSho KAMBAYASHI, Yoshikazu KAWAI
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Abstract
The pluripotent stem cell culture environment is appropriately controlled to improve production efficiency, and to suppress cell death when passaging pluripotent stem cells from suspension culture. Pluripotent stem cells subjected to suspension culture in a perfusion mode under appropriate medium perfusion conditions are passaged in the subsequent culture step, thereby improving passage efficiency. By strictly controlling the amount of medium perfused in line with the progress of culture of pluripotent stem cells and/or adjusting the amount of carbon dioxide gas supplied in line with the progress of culture of pluripotent stem cells, culture efficiency and productivity can be remarkably improved.
Claims24
A method for producing a pluripotent stem cell population, comprising:
- a suspension culture step of performing a suspension culture of pluripotent stem cells in a liquid medium in a perfusion mode;
- and
- a passage step of passaging the pluripotent stem cells subjected to the suspension culture,
- wherein the suspension culture step comprises controlling the amount of medium perfused per unit time in a range of 1% to 100% of a culture volume.
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Dependencies are inferred from the claim text.