Cell system and methods of use
AssigneeMelliCell, Inc.
InventorsAaron L. GLIEBERMAN, Benjamin D. POPE, David C. BOUCK
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Abstract
In some embodiments, the present disclosure provides cell systems that include arrangements of cells maintained by various separation systems and/or support layers for culture and differentiation of cells as well as related methods of use and preparation.
Claims101
A cell separation system comprising:
- (i) a substrate;
- and
- (ii) a cell separation feature in or on the substrate for spatially separating individual cells.
A cell support member comprising a porous material for maintaining a position of a cell on a substrate, the cell support member configured for placement over the cell to prevent passage of the cell through the porous material while allowing fluid communication through the porous material.
A placement system for engaging a cell support member with a cell separation system comprising:
- (i) a punch having at least one punch head with a distal face;
- (ii) an actuating system for engaging the at least one punch head with the cell support member to disengage the cell support member from a cell support layer, and to engage the cell support member with the cell separation system;
- and
- (iii) optionally, an alignment system for aligning the punch with the cell support member.
A method of forming a cell support member comprising the steps of:
- (i) providing a sheet of material;
- (ii) cutting at least one hole in the sheet of material;
- (iii) forming a support member body from the sheet of material by cutting an outer perimeter of the support member body around the hole;
- and
- (iv) applying a porous material over the hole.
A method of preparing a cell system, the method comprising:
- (i) providing a cell separation system comprising a cell separation structure on a substrate;
- and
- (ii) associating a cell with an attachment area on the substrate.
Dependencies are inferred from the claim text.