Method for improving product titer in a cell and a cell media for improving the same
AssigneeValitacell Limited
InventorsDevika KALSI, David JAMES, Ben THOMPSON, Jerry CLIFFORD
Life of this patent
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Abstract
A cell culture media comprising an effective amount of 3-Thiopheneacetic acid (3TAA).
Claims10
-7. (canceled)
A method for increasing product titer in a cell culture system, the method comprising the steps of:
- (a) incubating a cell in the producer cell culture media comprising an effective amount of 3-Thiopheneacetic acid (3TAA) for a period of time in the cell culture system;
- (b) collecting the cell or cell culture media or both from the cell culture system;
- and
- (c) harvesting the product produced by the cell in the cell culture system.
A method for increasing protein production of a protein-producing cell in a cell culture system, the method comprising the steps of:
- (a) incubating the protein-producing cell in the producer cell culture media comprising an effective amount of 3-Thiopheneacetic acid (3TAA) for a period of time;
- (b) collecting the producer cell or producer cell culture media, or both, from the system;
- and
- (c) harvesting the protein from the producer cell or producer cell culture media, or both.
A method of determining whether the producer cell culture media comprising an effective amount of 3-Thiopheneacetic acid (3TAA) increases product titer in a cell culture system when compared to product titer when using the producer cell culture media without 3TAA, the method comprising the steps of:
- incubating a cell with the producer cell culture media with and without 3TAA;
- determining the product titer of the cell in each media;
- and
- comparing the product titer of the cell in each media.
A use of an effective amount of 3-Thiopheneacetic acid (3TAA) in a producer cell culture media to increase production titer in a batch production process or in a fed batch process.
(canceled)
Dependencies are inferred from the claim text.