Method for suppressing production of degradation products
AssigneeKyowa Kirin Co., Ltd.
InventorsTsuyoshi YAMAGUCHI, Mie FUKUDA, Hiroko ISHIKAWA, Ryuma NAGANO, Toshiyuki SUZAWA
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Abstract
An object of the present invention is to provide a culture method by which an LMWS amount is minimized while high productivity of a target protein is maintained. The present invention relates to a method for preventing generation of a degradation product (a low molecular weight species: LMWS) of a target protein. The method includes: a means for, in a cell culture process for producing the target protein at a high concentration in a culture medium, removing a reactive oxygen species in the culture medium.
Claims32
A method for preventing generation of a degradation product (a low molecular weight species:
- LMWS) of a target protein, the method comprising:
- producing the target protein at a high concentration in a culture medium during a cell culture process, and removing a reactive oxygen species in the culture medium during said cell culture process.
The method according to claim 12, wherein the amount of the LMWS generated is reduced as compared with a cell culture process not including the removing of the reactive oxygen species.
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A method for producing a target protein with a reduced LMWS amount at a high concentration in a culture medium, the method comprising:
- removing a reactive oxygen species in the culture medium during a cell culture process.
The method according to claim 2324, wherein the amount of the LMWS generated is reduced as compared with a cell culture process not including the removing of the reactive oxygen species.
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Dependencies are inferred from the claim text.