Systems, microorganisms, or methods for waste pet valorization
AssigneeWashington University
InventorsTae Seok Moon, Jinjin Diao, Yifeng Hu, Kirsten Davis
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Abstract
Among the various aspects of the present disclosure is the provision of methods, systems, and Rhodococcus sp. strains for the upcycling of polyethylene terephthalate) (PET). An aspect of the present disclosure provides for a system for waste PET valorization comprising: a microorganism capable of growing on PET hydrolysis products, such as PET hydrolysate. PET products from chemical hydrolysis, or alkaline hydrolysis products of PET as a carbon source.
Claims21
1-44. (canceled)
A system for waste polyethylene terephthalate (PET) valorization, the system comprising:
- an engineered Rhodococcus sp. strain capable of utilizing PET hydrolysate as a carbon source;
- and
- a medium comprising a PET hydrolysate, wherein the PET hydrolysate comprises at least one of terephthalic acid (TPA) and ethylene glycol (EG).
A method for generating a target product from waste polyethylene terephthalate (PET) valorization, the method comprising:
- providing an engineered Rhodococcus sp. strain capable of utilizing PET hydrolysate as a carbon source;
- and
- incubating the engineered Rhodococcus sp. Strain in a medium comprising a PET hydrolysate, wherein the PET hydrolysate comprises at least one of terephthalic acid (TPA) and ethylene glycol (EG).
An engineered Rhodococcus sp. strain transformed with an artificial DNA construct, wherein the artificial DNA construct comprises:
- (a) a promoter functional in the Rhodococcus sp. strain;
- and
- (b) at least one polynucleotide selected from:
- (i) a first polynucleotide comprising a nucleotide sequence encoding a first polypeptide having prenyltransferase—(CrtE), phytoene synthase—(CrtB), and phytoene desaturase—(CrtI) enzymatic activity);
- (ii) a second polynucleotide comprising a nucleotide sequence encoding a second polypeptide having 1-deoxyxylulose-5-phosphate synthase enzymatic activity;
- (iii) a third polynucleotide comprising a nucleotide sequence encoding a third polypeptide having isopentenyl pyrophosphate isomerase enzymatic activity;
- and
- (iv) a fourth polynucleotide comprising a nucleotide sequence encoding a fourth polypeptide having 1-deoxyxylulose-5-phosphate synthase and isopentenyl pyrophosphate isomerase enzymatic activity;
- and
- (c) a transcriptional termination sequence;
- and wherein, a crtL-b gene in the engineered Rhodococcus sp. strain is knocked out or knocked down.
Dependencies are inferred from the claim text.