Anti-fgfr2 antibodies and methods of use thereof

A nucleic acid molecule comprising a polynucleotide sequence that encodes a heavy chain variable region (HCVR) and/or a polynucleotide sequence that encodes a light chain variable region (LCVR) of a human monoclonal antibody or antigen-binding fragment thereof that binds to FGFR2, wherein the antibody or antigen-binding fragment thereof comprises:

• three heavy chain complementarity determining regions (CDRs) (HCDR1, HCDR2 and HCDR3) contained within the HCVR sequence set forth in SEQ ID NO:
• 2, SEQ ID NO:
• 22 or SEQ ID NO:
• 40;
• and
• three light chain CDRs (LCDR1, LCDR2 and LCDR3) contained within the LCVR sequence set forth in SEQ ID NO:
• 10, SEQ ID NO:
• 28 or SEQ ID NO:
• 44.

A composition comprising a first nucleic acid molecule and a second nucleic acid molecule, wherein:

• a) the first nucleic acid molecule comprises a polynucleotide sequence encoding an HCVR of a human monoclonal antibody or antigen-binding fragment thereof that binds to FGFR2, wherein the HCVR comprises an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 4, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 6, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 8;
• and the second nucleic acid molecule comprises a polynucleotide sequence encoding an LCVR of the human monoclonal antibody or antigen-binding fragment thereof that binds to FGFR2, wherein the LCVR comprises an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 12, an LCDR2 amino acid comprising the sequence of SEQ ID NO:
• 14, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:
• 16;
• or
• b) the first nucleic acid molecule comprises a polynucleotide sequence encoding an HCVR of a human monoclonal antibody or antigen-binding fragment thereof that binds to FGFR2, wherein the HCVR comprises an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 24, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 6, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 26;
• and the second nucleic acid molecule comprises a polynucleotide sequence encoding an LCVR of the human monoclonal antibody or antigen-binding fragment thereof that binds to FGFR2, wherein the LCVR comprises an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 30, an LCDR2 amino acid comprising the sequence of SEQ ID NO:
• 32, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:
• 34;
• or
• c) the first nucleic acid molecule comprises a polynucleotide sequence encoding an HCVR of a human monoclonal antibody or antigen-binding fragment thereof that binds to FGFR2, wherein the HCVR comprises an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 24, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 6, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 42;
• and the second nucleic acid molecule comprises a polynucleotide sequence encoding an LCVR of the human monoclonal antibody or antigen-binding fragment thereof that binds to FGFR2, wherein the LCVR comprises an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 46, an LCDR2 amino acid comprising the sequence of SEQ ID NO:
• 32, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:
• 34.

An expression vector comprising a first nucleic acid molecule and a second nucleic acid molecule, wherein:

• a) the first nucleic acid molecule comprises a polynucleotide sequence that encodes an HCVR comprising the amino acid sequence of SEQ ID NO:
• 2, and the second nucleic acid molecule comprises a polynucleotide sequence that encodes an LCVR comprising the amino acid sequence of SEQ ID NO:
• 10;
• or
• b) the first nucleic acid molecule comprises a polynucleotide sequence that encodes an HCVR comprising the amino acid sequence of SEQ ID NO:
• 22, and the second nucleic acid molecule comprises a polynucleotide sequence that encodes an LCVR comprising the amino acid sequence of SEQ ID NO:
• 28;
• or
• c) the first nucleic acid molecule comprises a polynucleotide sequence that encodes an HCVR comprising the amino acid sequence of SEQ ID NO:
• 40, and the second nucleic acid molecule comprises a polynucleotide sequence that encodes an LCVR comprising the amino acid sequence of SEQ ID NO:
• 44.