High-throughput methods for analyzing and affinity-maturing an antigen-binding molecule
Assignee10x Genomics, Inc.
InventorsWyatt James MCDONNELL, Michael John Terry STUBBINGTON, Katherine PFEIFFER
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Abstract
The present disclosure generally relates to compositions (e.g., nucleic acid constructs, vectors, plasmids, engineered cells, etc.) and methods using the same for selecting and/or making an antigen-binding molecule of interest (e.g., an immune receptor, an antibody or functional fragment thereof) with improved affinity to a target antigen.
Claims30
An nucleic acid construct comprising:
- a) a third nucleic acid sequence encoding an antigen-binding molecule of interest, and
- b) a nucleic acid sequence encoding an enzyme capable of mediating SHM, wherein the coding sequence for the SHM enzyme is operably linked to an inducible promoter, wherein the inducible promoter is induced by an immune response.
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A nucleic acid construct comprising one or more of the following:
- a) a first heterologous nucleic acid sequence encoding a self-targeting guide RNA (stgRNA) operably linked to a first promoter,
- b) a second heterologous nucleic acid sequence encoding a CRISPR-associated (Cas) protein operably linked to a second promoter, wherein the second promoter is induced by an immune response;
- and
- c) a third nucleic acid sequence encoding an antigen-binding molecule of interest.
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Dependencies are inferred from the claim text.