Separation media and purification methods for carbohydrate containing molecules using the same

A method of isolating a target molecule from an isolation solution,

• the isolation solution comprising:
• an isolation solvent;
• and
• the target molecule comprising a free protein and the free protein comprising a carbohydrate,
• the method comprising:
• contacting the isolation solution with a separation media,
• the separation media comprising:
• a support substrate;
• and
• a plurality of separation ligands for formula SL1 or SL2
• wherein:
• Z is a separation group comprising an affinity group, the affinity group comprising a carbohydrate binding domain, a carbohydrate binging ligand, or both;
• and
• Rp1, Rp3, and Rp4 each independently comprise the reaction product of any one of RpA, RpB, RpC, RpD, RpE, RpF, RpG, RpH, RpI, RpJ, RpK, RpL, and RpM or an isomer thereof, wherein RpA, RpB, RpC, RpD, RpE, RpF, RpG, RpH, RpI, RpJ, RpK, RpL, and RpM are represented by:
• wherein U0, U1, U2, U3, U4, U5, U6, U7, U8, and U9 are each independently NH, O, or S;
• R is an organic group, H, or halogen;
• and
• Sp is a spacer comprising a divalent organic group.