# US 2024/0189263 A1 · Derivatized c60 molecules and methods and materials for using derivatized c60 molecules to reduce radiation injury > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2024/0189263 A1 - Document ID: US20240189263A1 - Status: Published application - Kind: A1 - Application number: 18/286,297 - Filed: 2022-06-24 - Published: 2024-06-13 - Earliest claimed: 2021-06-28 - Assignees: Mayo Foundation for Medical Education and Research; AGNI Medical LLC - Applicants: Mayo Foundation for Medical Education and Research; AGNI Medical LLC - Inventors: Sunil Krishnan; Yuri Mackeyev; Elena Vishnyakova - Web page: https://patentgrep.com/patent/US20240189263A1 ## CPC - A61K 31/047: having two or more hydroxy groups, e.g. sorbitol - A61K 31/165: having aromatic rings, e.g. colchicine, atenolol, progabide - A61P 39/00: General protective or antinoxious agents - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract This document provides derivatized C60 molecules (e.g., C60-serinol) and methods and materials for using derivatized C60 molecules (e.g., C60-serinol) to reduce radiation injury. For example, methods and materials for using derivatized C60 molecules (e.g., C60-serinol) to reduce, prevent, and/or mitigate radiation injury (e.g., radiation induced oxidative stress and/or DNA damage) to cells (e.g., human cells) or to a mammal (e.g., a human) during, for example, outer space missions are provided. ## Claims ### Claim 1 A method for prolonging survival of a mammal exposed to a lethal level of radiation, wherein said method comprises administering a composition comprising C60-serinol to said mammal, wherein said mammal survives longer than if said mammal was not administered said composition. ### Claim 2 (depends on 1) The method of claim 1, wherein said mammal is human. ### Claim 3 (depends on 1) The method of claim 1, wherein said lethal level of radiation is greater than 5 Gy. ### Claim at position 4 4-8. (canceled) ### Claim 9 A method for prolonging survival of a mammal expected to be exposed to a lethal level of radiation, wherein said method comprises administering a composition comprising C60-serinol to said mammal before said mammal is exposed to said lethal level of radiation, wherein said mammal survives longer than if said mammal was not administered said composition. ### Claim 10 (depends on 9) The method of claim 9, wherein said mammal is human. ### Claim 11 (depends on 9) The method of claim 9, wherein said lethal level of radiation is greater than 5 Gy. ### Claim at position 8 12-16. (canceled) ### Claim 17 A method for reducing the severity of radiation-induced injury, wherein said method comprises administering a composition comprising C60-serinol to a mammal exposed to a damaging level of radiation, wherein the severity of radiation-induced injury of said mammal is less than the severity of radiation-induced injury if said mammal was not administered said composition. ### Claim 18 (depends on 17) The method of claim 17, wherein said mammal is human. ### Claim 19 (depends on 17) The method of claim 17, wherein said damaging level of radiation is greater than 0.5 Gy. ### Claim at position 12 20-24. (canceled) ### Claim 25 A method for reducing the severity of radiation-induced injury, wherein said method comprises administering a composition comprising C60-serinol to a mammal prior to exposure to a damaging level of radiation, wherein said mammal is exposed to said damaging level of radiation, and wherein the severity of radiation-induced injury of said mammal is less than the severity of radiation-induced injury if said mammal was not administered said composition. ### Claim 26 (depends on 25) The method of claim 25, wherein said mammal is human. ### Claim 27 (depends on 25) The method of claim 25, wherein said damaging level of radiation is greater than 0.5 Gy. ### Claim at position 16 28-32. (canceled) ### Claim 33 A method for treating a mammal to reduce the number of reactive oxygen species in said mammal following radiation exposure, wherein said method comprises administering a composition comprising C60-serinol to said mammal before, during, or after exposure to a damaging level of radiation, wherein said mammal is exposed to said damaging level of radiation, and wherein the number of reactive oxygen species in said mammal following said radiation exposure is less than the number of reactive oxygen species in said mammal if said mammal was not administered said composition. ### Claim 34 (depends on 33) The method of claim 33, wherein said mammal is human. ### Claim 35 (depends on 33) The method of claim 33, wherein said damaging level of radiation is greater than 0.5 Gy. ### Claim at position 20 36-43. (canceled) ### Claim 44 A composition comprising C60-serinol. ### Claim 45 (depends on 44) The composition of claim 44, wherein said composition further comprises an aqueous or non-aqueous sterile injection solution. ### Claim 46 (canceled) ### Claim 47 A method of making C60-serinol, wherein said method comprises: (a) reacting serinol malonate tetraacetate with [60]fullerene in the presence of 1,8-diazabicyclo[5.4.0]undec-7-ene and perbromomethane in toluene to yield C60-serinol acetate; and (b) reacting said C60-serinol acetate with hydrochloric acid in an aqueous solution of dioxane to yield C60-serinol. ### Claim 48 (depends on 47) The method of claim 47, wherein reacting serinol malonate tetraacetate with [60]fullerene comprises reacting serinol malonate tetraacetate and [60]fullerene in a 6:1 to 15:1 molar ratio. ### Claim 49 (canceled) ### Claim 50 (depends on 47) The method of claim 47, wherein said method comprises reacting serinol malonate with acetic anhydride to yield serinol malonate tetraacetate. ### Claim 51 (canceled)