Devices and methods for sample analysis with serial dilution
AssigneeCytoChip Inc.
InventorsWendian Shi, Yuan Gao, Yuzhe Ding
Life of this patent
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Abstract
Devices and methods for analyzing a sample are disclosed. In various embodiments, the present disclosure provides devices and methods for preparing a serial dilution of a sample. In various embodiments, the present disclosure provides devices and methods for preparing a serial dilution of a sample and conducting sample analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device. The reader instrument device receives, operates, and/or actuates the cartridge device to prepare a serial dilution of a sample and conduct sample analysis.
Claims20
A device comprising a fluidic cartridge, wherein the fluidic cartridge comprises:
- a first chamber to receive a sample mixture 1;
- a second chamber to receive a sample mixture 2;
- a third chamber to receive a sample mixture 3;
- and
- a metering chamber for metering a first portion of the sample mixture 1, the metering chamber being connected to the first chamber;
- wherein:
- the first chamber is configured to mix a sample with a first reagent to form the sample mixture 1;
- a driving mechanism is applied to the fluidic cartridge to meter the first portion of the sample mixture 1 in the metering chamber;
- the second chamber is configured to mix the first portion of the sample mixture 1 with a second reagent to form the sample mixture 2;
- and
- the third chamber is configured to mix a second portion of the sample mixture 1 with a third reagent to form the sample mixture 3.
A method comprising following steps:
- mixing a sample with a first reagent to form a sample mixture 1 in a first chamber;
- metering a first portion of the sample mixture 1 in a metering chamber by applying a driving mechanism to a fluidic cartridge;
- mixing the first portion of the sample mixture 1 with a second reagent to form a sample mixture 2 in a second chamber;
- and
- mixing a second portion of the sample mixture 1 with a third reagent to form a sample mixture 3 in a third chamber;
- wherein the fluidic cartridge comprises the first chamber, the second chamber, the third chamber, and the metering chamber.
Dependencies are inferred from the claim text.