Isothermal based-dual functional oligonucleotide including reporter dye, and quencher for isothermal nucleic acid amplification and measurement methods using same

A method for performing a loop-mediated isothermal nucleic acid amplification reaction (LAMP) for a real-time nucleic acid amplification fluorescence detection or the reverse transcription (RT)-LAMP reaction using an oligonucleotide in the isothermal, wherein the oligonucleotide is designed for use in a loop-mediated isothermal nucleic acid amplification (LAMP) reaction for a specific sequence of a target gene, wherein the oligonucleotide comprises two target gene sites, and one of the oligonucleotide sequence for the target gene in the 5′ end direction of two target gene sites except 5′-terminal sequence is positioned as a reporter dye, the sequence of one of the oligonucleotide sequence for the target gene in the 3′ direction except 3′ terminal sequence is positioned as a quencher, and the sequence in which the corresponding reporter dye or quencher is located is modified to Amino-C6 (or C2)-deoxythymidine or Amino-C6-deoxyribose and wherein there is within a 21-33 mer interval between the reporter dye and the quencher.