Anastasis biosensor caspase tracker
AssigneeThe Johns Hopkins University
InventorsHo Lam Hogan Tang, Ho Man Holly Tang
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Abstract
The present invention relates to the field of anastasis, i.e., the process of reversal of apoptosis. More specifically, the present invention provides methods and compositions useful for studying anastasis. In one embodiment, a tracking construct of the present invention comprises Lyn11-NES-ERT2-DEVD-rtTA-3×FLAG-DEVD-ERT2-NES. In another embodiment, a construct comprises Lyn11-NES-DEVD-rtTA-3×FLAG. In a further embodiment, a construct comprises ERT2-DEVD-rtTA-3×FLAG-DEVD-ERT2.
Claims19
A polynucleotide comprising SEQ ID NO:1.
A polynucleotide comprising SEQ ID NO:3.
A polynucleotide comprising SEQ ID NO:27.
A polynucleotide comprising SEQ ID NO:29.
A polynucleotide comprising SEQ ID NO:31.
A polynucleotide comprising SEQ ID NO:33.
A polynucleotide comprising SEQ ID NO:35.
A polynucleotide comprising SEQ ID NO:37.
A polynucleotide comprising SEQ ID NO:39.
A polypeptide comprising SEQ ID NO:2.
A polypeptide comprising SEQ ID NO:4.
A polypeptide comprising SEQ ID NO:28.
A polypeptide comprising SEQ ID NO:30.
A polypeptide comprising SEQ ID NO:32.
A polypeptide comprising SEQ ID NO:34.
A polypeptide comprising SEQ ID NO:36.
A polypeptide comprising SEQ ID NO:38.
A polypeptide comprising SEQ ID NO:40.
Dependencies are inferred from the claim text.