Cell-free dna methylation test
AssigneeUniversity of Southern California
InventorsBudur SALHIA, Gerald Christopher GOODEN
Life of this patent
Estimating the term…
Abstract
The disclosure provides for certain assays and methods of determining the presence or absence of ovarian cancer, the severity of ovarian cancer, the histological subtype of ovarian cancer, or the susceptibility to ovarian cancer by examining the methylation levels of certain target genomic regions.
Claims27
A method for determining whether a subject is likely to have or develop epithelial ovarian cancer in a subject comprising:
- (a) measuring the level of nucleic acid methylation of a plurality of target genomic region listed in Table 1 from a cell-free nucleic acid sample from the subject;
- (b) comparing the level of nucleic acid methylation of the plurality of target genomic region in the sample to the level of nucleic acid methylation of the plurality of target genomic regions in a sample isolated from a cancer-free subject, a cancer-free reference standard, or a cancer-free reference cutoff value;
- (c) determining that the subject is like to have or develop epithelial ovarian cancer based on a change in the level of nucleic acid methylation in the plurality of target genomic regions in the sample derived from the subject, wherein the change is greater or less than the level of nucleic acid methylation of the target genomic regions in the sample isolated from a cancer-free subject, a normal reference standard, or a normal reference cutoff value.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method for detecting high grade serous epithelial ovarian cancer in a subject comprising:
- (a) measuring the level of nucleic acid methylation of a plurality of target genomic region listed in Table 1 from a cell-free nucleic acid sample from the subject;
- (b) comparing the level of nucleic acid methylation of the plurality of target genomic region in the sample to the level of nucleic acid methylation of the plurality of target genomic regions in a sample isolated from a cancer-free subject, a cancer-free reference standard, or a cancer-free reference cutoff value;
- (c) determining that the subject has high grade serous epithelial ovarian cancer based on a change in the level of nucleic acid methylation in the plurality of target genomic regions in the sample derived from the subject, wherein the change is greater or less than the level of nucleic acid methylation of the target genomic regions in the sample isolated from a cancer-free subject, a normal reference standard, or a normal reference cutoff value.
A method for differentiating high grade serous epithelial ovarian cancer from non-high grade serous epithelial cancer in a subject comprising:
- (a) measuring a level of nucleic acid methylation of a plurality of target genomic region listed in Table 1 from a cell-free nucleic acid sample from the subject;
- (b) comparing the level of nucleic acid methylation of the plurality of target genomic region in the sample to a level of nucleic acid methylation of the plurality of target genomic regions in a sample isolated from a non-high grade serous epithelial ovarian cancer subject.;
- (c) determining that the subject has high grade serous epithelial ovarian cancer based on a change in the level of nucleic acid methylation in the plurality of target genomic regions in the sample derived from the subject, wherein the change is greater or less than the level of nucleic acid methylation of the target genomic regions in the sample isolated from a non-high grade serous epithelial ovarian cancer subject.
Dependencies are inferred from the claim text.