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US 2024/0182977 A1Published application
Methods for early detection of cancer
AssigneeGuardant Health, Inc.
InventorsStefanie Ann Ward MORTIMER, AmirAli TALASAZ, Darya CHUDOVA, Helmy ELTOUKHY
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Abstract
Disclosed herein are methods, compositions, and devices for use in the early detection of cancer. The methods include preparing cell-free nucleic acid molecules from a subject for sequencing, sequencing a panel of regions in the cell-free nucleic acid molecules, and detecting one or more markers that are indicative of a cancer.
Claims30
A method for detecting a presence or absence of residual disease in a subject, the method comprising:
- (a) enriching cell-free deoxyribonucleic acid (cfDNA) molecules or amplicons thereof from a sample of the subject using sequence capture for a sequencing panel of genes or genomic regions to generate the sequencing panel, wherein the sequencing panel:
- (i) is at least 150kb,
- (ii) comprises a plurality of genes or genomic regions having CpG islands which are differentially methylated regions,
- (iii) comprises a plurality of genes or genomic regions known to be associated with one or more cancers, wherein the subject previously received a treatment for cancer and the sample was obtained from the subject after the treatment;
- (b) sequencing a plurality of enriched cfDNA from the sequencing panel to generate sequencing data;
- and
- (c) determining methylation profiles of the cfDNA molecules and detecting a presence or absence of one or more genetic variants in the cfDNA molecules from the sequencing data to detect the presence or absence of residual disease in the subject.
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Dependencies are inferred from the claim text.