Immune cell quantification
AssigneeAcademisch Ziekenhuis Leiden (H.O.D.N. Lumc)
InventorsWillem H. ZOUTMAN, Pieter A. VAN DER VELDEN, Rogier J. NELL
Life of this patent
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Abstract
The present invention provides novel methods and kits for quantifying immune cells, specifically B-cells and T-cells, in a sample. The methods and kits may be used to monitor disease progression. They may also be used to determine the effect of a medicament used in the treatment of a disease. They may also be used to determine disease prognosis. They may also be used to diagnose disease.
Claims46
A method for determining the VDJ rearranged human T-cell fraction in a sample, the method comprising:
- a) quantifying, in the sample, the amount of:
- a diploid reference DNA marker;
- a TCR DNA marker selected from an intergenic region between D62 and D63 on chromosome 14q11.2 or an intergenic region between D61 and 161.1 on chromosome 7q34;
- and
- a DNA regional corrector of the TCR marker;
- and
- b) determining the VDJ rearranged human T-cell fraction in the sample based on the quantification obtained in step a), wherein the VDJ rearranged T-cell fraction is determined as:
- T-cell fraction=([DNA regional corrector]−[TCR DNA marker])/[diploid reference DNA marker].
A method for determining the VDJ rearranged human B-cell fraction in a sample, the method comprising:
- a) quantifying, in the sample, the amount of:
- a diploid reference DNA marker;
- and
- a B-cell DNA marker comprising an intergenic sequence between IGHD7-27 and lain at chromosome 14q32.33;
- and
- b) determining the VDJ rearranged human B-cell fraction in the sample based on the quantification obtained in step a), wherein the VDJ rearranged human B-cell fraction is determined as:
- B-cell fraction=1−([B-cell DNA marker]/[diploid reference DNA marker]).
A method for determining the VDJ rearranged human B-cell fraction in a sample, the method comprising:
- a) quantifying, in the sample, the amount of:
- a diploid reference DNA marker;
- a B-cell DNA marker comprising an intergenic sequence between IGHD7-27 and lain at chromosome 14q32.33;
- and
- a DNA regional corrector of the B-cell DNA marker;
- b) determining the VDJ rearranged human B-cell fraction in the sample based on the quantification obtained in step a),
- wherein the VDJ rearranged human B-cell fraction is determined as:
- B-cell fraction=([DNA regional corrector]−[B-cell DNA marker])/[diploid reference DNA marker].
A method for determining the class-switched human B-cell fraction in a sample, the method comprising:
- a) quantifying, in the sample, the amount of:
- a diploid reference DNA marker;
- and
- a class-switched B-cell DNA marker comprising a sequence of IGHD at chromosome 14q32.33;
- and
- b) determining the class-switched human B-cell fraction in the sample based on the quantification obtained in step a), wherein the class-switched human B-cell fraction is determined as:
- class-switched fraction=1−([class-switched B-cell DNA marker]/[diploid reference DNA marker]).
A method for determining the class-switched human B-cell fraction in a sample, the method comprising:
- a) quantifying, in the sample, the amount of:
- a diploid reference DNA marker;
- a class-switched B-cell DNA marker comprising a sequence of IGHD at chromosome 14q32.33;
- and
- a DNA regional corrector of the class-switched B-cell DNA marker;
- and
- b) determining the class-switched human B-cell fraction in the sample based on the quantification obtained in step a), wherein the class-switched human B-cell fraction is determined as:
- class-switched fraction=([DNA regional corrector]−[class-switched B-cell DNA marker])/[diploid reference DNA marker].
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Dependencies are inferred from the claim text.