Method for monitoring or predicting whether a patient suffering from obesity is responding or will respond to a very-low-calorie ketogenic diet (vlckd)

In vitro method, according to any of the previous claims, for monitoring or predicting whether a patient suffering from obesity is responding or will respond to a VLCKD giving rise to both weight loss and nutritional ketosis induction characterized by low fat consumption, which comprises determining the methylation status of at least a gene selected from Table 2, wherein a statistically significant variation or deviation of level of methylation, as compared with a pre-established level of methylation, is an indication that the subject is responding or will respond to a VLCKD.

In vitro method, according to any of the previous claims, wherein an overall hypomethylation of the genes is observed when the patient is responding or will respond to the treatment with a VLCKD.

In vitro method, according to any of the previous claims, wherein the methylation status detection is conducted by means of a technique selected from the group consisting of:

• methylation specific PCR, bisulphite sequencing, techniques based on restriction-digestion, pyrosequencing, assay ChIP-on-chip, differential conversion, differential restriction and/or differential weight of site(s) methylated.

In vitro method, according to any of the previous claims, characterized in that it is carried out in blood leucocytes.

In vitro use of the methylation status of at least a gene or CpG selected from Table S2, Table S3 or Table S4 for monitoring or predicting whether a patient suffering from obesity is responding or will respond to a VLCKD.