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US 2024/0182971 A1Published application
Method for diagnosing dry mouth using biomarkers
OwnerBhuvaneswari GURUMURTHY, Donghui WU +2inventors
InventorsBhuvaneswari GURUMURTHY, Donghui WU, Michael FITZGERALD, David WONG
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Abstract
The present invention describes a method for the detection and monitoring of xerostomia in a subject using biomarkers.
Claims19
A method of diagnosing xerostomia in a subject, comprising:
- (a) isolating a biological sample from the subject;
- (b) detecting a level of expression and/or DNA methylation of at least one gene selected from genes listed in Tables 1, 2, 4, and 5 in the saliva sample from the subject;
- (c) comparing the level of expression and/or DNA methylation of the at least one gene in the sample to a level of expression and/or DNA methylation in a reference,
- wherein an increased or decreased level of expression and/or DNA methylation of the at least one gene in the sample compared to the level in the reference identifies the subject having xerostomia and wherein the biological sample is biopsied parotid gland or saliva.
1 more element
A method of monitoring the response to a xerostomia treatment in a subject, comprising
- (a) isolating a biological sample from the subject after the treatment is initiated;
- (b) detecting a level of expression and/or DNA methylation of at least one gene selected from genes listed in Tables 1, 2, 4, and 5 in the biological sample from the subject;
- (c) comparing the level of expression and/or DNA methylation of the at least one gene in the sample to a level of expression and/or DNA methylation in a reference,
- wherein an increased or decreased level of expression and/or DNA methylation of the at least one gene in the sample compared to the level in the reference indicates that the subject is responsive to the treatment and wherein the biological sample is biopsied parotid gland or saliva.
1 more element
A kit for diagnosing and/or monitoring xerostomia, comprising at least one reagent for the determination of the level of mRNA or polypeptide or the level of DNA methylation of at least one gene selected from genes listed in Tables 1, 2, 4, and 5.
Dependencies are inferred from the claim text.