Methods and compositions for detection of tumor dna
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, The General Hospital Corporation
InventorsViktor A. Adalsteinsson, Nir Hacohen, Sangeeta N. Bhatia, Sahil Patel, Zhenyi An
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Abstract
This disclosure provides a method and compositions for substantially increasing the concentration of DNA in macrophages of a patient. By administering to a patient one or more agents which prevent the activity of deoxyribonucleases within lysosomes of macrophages, degradation of DNA phagocytosed by macrophages is temporarily blocked, permitting its accumulation. This strategy has the potential to enhance the detection of genetic biomarkers from cells typically phagocytosed by macrophages, such as tumor cells, and thus has applications for early or residual detection of cancer.
Claims116
A method for increasing the concentration of tumor DNA in a macrophage or population thereof, comprising administering to a subject an effective amount of an agent that results in an increase in the accumulation of tumor DNA in the macrophage or population thereof in the subject.
A method for increasing the recovery of tumor DNA from a macrophage or population thereof, comprising:
- (a) administering to a subject an effective amount of an agent that increases the accumulation of tumor DNA in a macrophage or population thereof in a subject;
- and
- (b) obtaining the tumor DNA from the macrophage or population thereof.
A method for enhancing tumor detection in a tissue, comprising administering an agent that increases the accumulation of tumor DNA in a macrophage or population thereof in the tissue, and evaluating the accumulated tumor DNA, thereby detecting the tumor.
A pharmaceutical composition for increasing the concentration of tumor DNA, comprising:
- (a) an agent capable of increasing the concentration of tumor DNA in a macrophage or population thereof;
- and
- (b) a pharmacologically acceptable excipient.
A kit for increasing the concentration of phagocytosed DNA in a population of macrophages in a subject, comprising:
- (a) a pharmaceutical composition comprising an agent capable of increasing the concentration of tumor DNA in the macrophage or population thereof;
- (b) optionally a device for administering said composition;
- and
- (c) instructions for administering the composition.
A method for detecting a tumor, comprising collecting a macrophage or population thereof which are associated with the tumor, and evaluating the tumor DNA that is accumulated by the macrophage or population thereof, thereby detecting the tumor.
Dependencies are inferred from the claim text.