Enzyme composition
AssigneeDsm IP Assets B.V.
InventorsMaaike APPELDOORN, Wouter KROES
Life of this patent
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Abstract
The application relates to an enzyme composition, a process for the preparation thereof and the use of the enzyme composition in enzymatic hydrolysis.
Claims15
An enzyme composition comprising glucoamylase (GA) and cellobiohydrolase I (CBHI), wherein glucoamylase activity is present at a fraction relative to glucoamylase activity and cellobiohydrolase I activity as defined by RGA, and wherein cellobiohydrolase I activity is present at a fraction relative to cellobiohydrolase I activity and glucoamylase activity as defined by RCBHI, wherein RCBHI is from 0.42 to 0.97 and RGA is from 0.03 to 0.58.
A process for preparation of a sugar from cellulosic material comprising the steps of:
- a) hydrolysing the cellulosic material with an enzyme composition to obtain the sugar, and
- b) optionally, recovering the sugar,
- wherein the enzyme composition comprises glucoamylase and cellobiohydrolase I and wherein glucoamylase activity is present at a fraction relative to glucoamylase activity and cellobiohydrolase I activity as defined by RGA, and wherein cellobiohydrolase I activity is present at a fraction relative to cellobiohydrolase I activity and glucoamylase activity as defined by RCBHI, wherein RCBHI is from 0.42 to 0.97 and RGA is from 0.03 to 0.58.
A process for producing a fermentation product from a cellulosic material, which process comprises:
- a) hydrolysing the cellulosic material with an enzyme composition to obtain a sugar,
- b) fermenting the obtained sugar by contacting the obtained sugar with a fermenting microorganism to produce the fermentation product, and
- c) optionally, recovering the fermentation product,
- wherein the enzyme composition comprises glucoamylase and cellobiohydrolase I and wherein glucoamylase activity is present at a fraction relative to glucoamylase activity and cellobiohydrolase I activity as defined by RGA, and wherein cellobiohydrolase I activity is present at a fraction relative to cellobiohydrolase I activity and glucoamylase activity as defined by RCBHI, wherein RCBHI is from 0.42 to 0.97 and RGA is from 0.03 to 0.58.
Dependencies are inferred from the claim text.