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US 2024/0182935 A1Published application
Method of producing & processing diamines
AssigneeGenomatica, Inc.
InventorsLauri H. Suominen, Connor J. Galleher, Michael Japs, Mark J. Burk, Cara Tracewell
Life of this patent
Estimating the term…
Abstract
Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
Claims20
1-217. (canceled)
A method for diamine (DA) production comprising steps of:
- a) culturing a genetically engineered microorganism in a medium under suitable conditions and for a sufficient period of time form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, or DA bis-bicarbonate, and/or DA carbamate or DA biscarbamate, wherein carbon dioxide, carbonate, bicarbonate or carbonic acid predominantly control pH of the medium;
- b) converting at least one or more of the DA carbonate, DA bicarbonate, or DA bis-bicarbonate, and/or DA carbamate or DA biscarbamate, into DA free base and carbon dioxide;
- and;
- c) isolating the DA free base.
1 more element
A method for diamine (DA) production comprising the steps of:
- a) culturing a genetically engineered microorganism in a medium under suitable conditions and for a sufficient period of time to form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, DA bis-bicarbonate, DA carbamate or DA biscarbamate wherein percent dissolved inorganic carbon (DIC) in the medium is greater than or equal to 40%;
- and the DIC is determined by the formula:
- DIC/TDCA×100
- wherein TDCA is the Total Dissolved Counter Anions and is the sum of DIC and other anions;
- b) converting the DA carbonate, DA bicarbonate, DA bis-bicarbonate, DA carbamate or DA biscarbamate into DA free base and carbon dioxide;
- and
- c) isolating the DA free base.
4 more elements
A method for diamine (DA) production comprising the steps of:
- a) culturing a genetically engineered microorganism in a medium under suitable conditions and for a sufficient period of time to form a cultured medium comprising one or more of DA carbonate, DA bicarbonate, DA bis-bicarbonate (DA Carbonates) and/or DA carbamate or DA biscarbamate (DA Carbamates) in the medium, wherein at least 40% of diamine species in the medium comprises one or more of DA carbonate, DA bicarbonate, DA bis-bicarbonate, DA carbamate or DA biscarbamate;
- b) converting the DA Carbonates and/or DA Carbamates into DA free base and carbon dioxide;
- and
- c) isolating the DA free base.
1 more element
Dependencies are inferred from the claim text.