Method of ethanol fermentation with cellulose at high-solids loading

A method of ethanol fermentation with cellulose at high-solids loading, comprising the following steps:

• 1) pretreatment:
• mixing a lignocellulose A pretreated with a NaOH-enhanced ethanol solution and a lignocellulose B pretreated with a AlCl3-enhanced ethanol solution in a ratio of (2:8) to (4:6) to obtain a mixed cellulose having a moisture content of 25 to 60%, and mixing the mixed cellulose and water according to a mass ratio of absolute dry cellulose to water of (7-10):100 to obtain a mixed cellulose solution;
• 2) sterilization treatment:
• adding nutrient salts to the mixed cellulose solution in step 1) for sterilization to obtain a sterilized solution;
• 3) saccharification and fermentation:
• adding cellulase and a yeast activation solution to the sterilized solution, and then performing simultaneous saccharification and fermentation, wherein a total time of simultaneous saccharification and fermentation is 24 to 200 h;
• 4) material supplementation treatment:
• supplementing a sterilized mixed cellulose to a fermentation tank in batches or continuously within 6 to 24 h after start of fermentation, wherein after a completion of material supplementation, a mass ratio of absolute dry cellulose to water is (30-45):100, and a final mixing ratio of the lignocellulose A to the lignocellulose B is (5:5) to (6.5:3.5);
• wherein materials are supplemented in batches:
• the material supplementation is performed for a first time after the start of fermentation for 5 to 7 h, and a ratio of the lignocellulose A to the lignocellulose B in the first material supplementation is (4:6) to (6:4);
• the material supplementation is performed for a second time after the start of fermentation for 11 to 13 h;
• and the material supplementation is performed for the last time after the start of fermentation for 22 to 24 h.