Short multi-repeat rna targeting gene silencing
AssigneeKansas State University Research Foundation
InventorsDavid E. Cook, Veerendra Sharma, Sandeep Marla, Geoffrey Morris
Life of this patent
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Abstract
Short multi-repeat RNA targeting constructs for manipulating RNA targets and obtaining variable levels of gene silencing, and methods of using the same to silence RNA targets in mammals, insects, plants, and fungus. The constructs comprise two or more distinct guide nucleotide sequences (in the absence of CRISPR nuclease) that are complementary to one or more RNA targets, wherein each guide nucleotide sequence consists of a single-stranded antisense nucleotide fragment of 100 nt or less.
Claims29
Short multi-repeat RNA targeting constructs for manipulating one or more RNA targets, said constructs comprising two or more distinct guide nucleotide sequences that are complementary to one or more RNA targets, wherein each guide nucleotide sequence consists of a single-stranded antisense nucleotide fragment of 100 nt or less.
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