Interpenetrating network microbial hydrogel with natural polysaccharide and protein and preparation method thereof

Abstract

The present disclosure belongs to the technical field of biomaterials and microbial immobilization, and relates to an interpenetrating network (IPN) microbial hydrogel with natural polysaccharides (PS) and proteins (PN) and a preparation method thereof. The natural PS of the hydrogel is selected from alginates, and the natural PN is selected from fibrous protein silk fibroin (SF) or methacrylated SF (SilMA). The natural PS and PN mimic extracellular PS and PN of aerobic granular sludge (AGS). Components in the hydrogel are uniformly mixed and each crosslinked to form an IPN. The natural PS undergoes ionic crosslinking, the SF in the natural PN undergoes self-assembly to form physical crosslinking, or the SilMA undergoes photocrosslinking. Loaded microbes uniformly adhere to a structure of the hydrogel. According to the preparation method of the hydrogel, a hydrogel precursor solution is sonicated and mixed with microbes, followed by bioprinting combined with crosslinking.

Dependencies are inferred from the claim text.