Prenyltransferase and a transgenic cell, tissue, and organism comprising same
AssigneeYeda Research and Development Co. Ltd.
InventorsAsaph AHARONI, Prashant SONAWANE, Adam JOZWIAK, Paula BERMAN, Luis DE-HARO
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Abstract
The present invention provides polynucleotide sequences derived from Helichrysum umbraculigerum and encoding a protein or a plurality thereof belonging to the prenyltransferase (PT) family. Further provided are an artificial nucleic acid molecule including the polynucleotide, a transgenic cell, tissue, or plant including same.
Claims32
An isolated DNA molecule comprising a nucleic acid sequence having at least 91% homology to SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, or any combination thereof.
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-14. (canceled)
-18. (canceled)
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A method for synthesizing a compound represented by Formula II:
- wherein:
- R1 is selected from the group consisting of:
- C1-C8 alkyl, and alpha-unsaturated phenylalkyl carboxylic acid, and R2 is OH;
- or (ii) R1 is OH and R2 is selected from the group consisting of:
- C1-C8 alkyl, and alpha-unsaturated phenylalkyl carboxylic acid, wherein R3 is a prenyl group, and wherein R4 is hydrogen or a prenyl group, the method comprising:
- a. providing a cell comprising an artificial vector comprising a nucleic acid sequence having at least 91% homology to SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or SEQ ID NO:
- 11;
- and
- b. culturing said cell from step (a) such that a protein encoded by said artificial vector is expressed,
- thereby synthesizing the compound represented by Formula II.
-31. (canceled)
A method for synthesizing a compound represented by Formula II:
- wherein:
- R1 is selected from the group consisting of:
- C1-C8 alkyl, and alpha-unsaturated phenylalkyl carboxylic acid, and R2 is OH;
- or (ii) R1 is OH and R2 is selected from the group consisting of:
- C1-C8 alkyl, and alpha-unsaturated phenylalkyl carboxylic acid, wherein R3 is a prenyl group, and wherein R4 is hydrogen or a prenyl group, the method comprising contacting a substrate molecule with an effective amount of a protein comprising an amino acid sequence with at least 92% homology to SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, or SEQ ID NO:
- 22, wherein said substrate molecule is represented by Formula I:
- wherein:
- (i) R1 is selected from the group consisting of:
- C1-C8 alkyl, and alpha-unsaturated phenylalkyl carboxylic acid;
- and R2 is OH;
- or (ii) R1 is OH and R2 is selected from the group consisting of:
- C1-C8 alkyl, and alpha-unsaturated phenylalkyl carboxylic acid, thereby synthesizing the compound represented by Formula II, and optionally wherein said contacting is in a cell-free system.
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-36. (canceled)
-44. (canceled)
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Dependencies are inferred from the claim text.