Tumor cell suspension cultures and related methods
AssigneeBeijing Percans Oncology Co., Ltd.
InventorsYiyou CHEN, Zhen YANG, Yihong ZHANG
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Abstract
Provided are suspension-based cell culture systems and media for the timely and efficient proliferation of human tumor cell clusters from a patient, and related methods of evaluating the potential responsiveness of the tumor cells and the patient to one or more therapeutic agents.
Claims29
A medium suspension, comprising a population of human tumor cell clusters from a human patient, wherein the medium suspension provides at least about 10% proliferation of the tumor cell clusters within about 14 days.
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The medium suspension of claim 5, comprising one or more components selected from DMEM/F-12, Wnt3A, basic fibroblast growth factor (bFGF), insulin, transferrin, bovine serum albumin (BSA), cholesterol, B-cell activating factor (BAFF), and 2-mercaptoethanol.
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21-24. (canceled)
Dependencies are inferred from the claim text.