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AssigneeThe Salk Institute for Biological Studies
InventorsHaisong LIU, Ronghui LI, Hsin-Kai LIAO, JUAN CARLOS IZPISUA BELMONTE
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Compositions for generating a pancreatic beta-like cell population from a population of undifferentiated cells and methods of use thereof, are provided. The method is an 8 stage process interrupted by a priming step, and it includes; using a chemically defined protocol for the efficient generation of pancreatic progenitors (PPs); improved assembly PPs into 3D clusters; a priming step which uses a chemical/factor cocktail (PP-10C) to maintain 3D-PPs status and enhances their potential to differentiate into β cells ;and a 3-step differentiation protocol using select chemical cocktails that efficiently converts PP-10C-treated 3D-PPs into functional β cells. The disclosed methods result in a population of functional β cells which expresses pancreatic cell markers selected from the group of c-peptide, the transcription factors NKX6.1, PDX1, PAX6, NEUROD1 and are INS+, and which do not express substantial levels Glucagon (GCG). The functional β cells can be used to treat conditions such as diabetes.
A method of generating cells of pancreatic beta lineage from pluripotent stem cells comprising:
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