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AssigneeUniversidad Nacional Autonoma De Mexico
InventorsMaria Gloria SOLDEVILA MELGAREJO, Evelyn Katy ALVAREZ SALAZAR, Josefina ALBERU GOMEZ, Arimelek CORTES HERNANDEZ
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A methodology to obtain in vitro large numbers of human induced regulatory T cells with specificity to the donor antigen, with a phenotype and stable suppressor function in the presence of pro-inflammatory cytokines, through of co-cultures of monocyte-derived dendritic cells and T cells “naïve”, both from genetically unrelated individuals (donor and recipient) is disclosed. The cells obtained with the present method are of CD4, CD25, CTLA-4 and FOXP3+ phenotype and show a specific suppressor function on donor antigen-specific T lymphocytes. These cells maintain their phenotype and stable suppressive function in presence of pro-inflammatory cytokines TNF-α and IL-6. The stability and the number obtained make them candidates as therapeutic tools for transplantation.
A method for in vitro generation and expansion of regulatory T cells comprising a subpopulation of induced regulatory human T cells comprising a phenotype CD25+CTLA-4+FOXP3+, allospecific and stable in the presence of proinflammatory cytokines, the method comprising at the 6th week of expansion, reaching an expansion of 23×104 times the initial number, wherein 90% of cells are CD25+CTLA-4+FOXP3+, and giving rise to 4,600 million allospecific iTregs from 20,000 naïve T cells.
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