Cell-culture bioreactors
AssigneeOrgenesis Inc.
InventorsMichael FAINSHTEIN, Nurislam GUBAEV, Vered CAPLAN
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Abstract
A bioreactor is provided, comprising a housing extending along a vertical axis and defining an internal cavity therewithin, top and bottom covers at opposite ends of the housing, and a plurality of ports, each configured to facilitate fluid communication between the internal cavity and the exterior of the bioreactor. The internal cavity has a frustoconical shape, the frustoconical shape being formed such that horizontal cross-sections are coaxial about a line which is angled with respect to the vertical axis.
Claims43
A bioreactor comprising:
- a housing extending along a vertical axis and defining an internal cavity therewithin, and top and bottom covers at opposite ends of the housing;
- and
- a plurality of ports, each configured to facilitate fluid communication between the internal cavity and the exterior of the bioreactor;
- wherein the internal cavity has a frustoconical shape, said frustoconical shape being formed such that horizontal cross-sections are coaxial about a line which is angled with respect to the vertical axis.
The bioreactor according to any one of the preceding claims, the housing comprising a vertically extending window.
The bioreactor according to any one of the preceding claims, wherein at least some of the ports are configured to facilitate selective fluid communication between the internal cavity and the exterior of the bioreactor.
The bioreactor according to any one of the preceding claims, at least one of the ports constituting a liquid injection port facilitating injection of a liquid into the internal cavity.
The bioreactor according to any one of the preceding claims, at least one of the ports constituting a collection port configured to be connected to an external container.
The bioreactor according to any one of the preceding claims, at least one of the ports constituting a gas supply port.
The bioreactor according to any one of the preceding claims, at least one of the ports constituting a needleless access port.
The bioreactor according to any one of the preceding claims, at least one of the ports constituting a liquid supply port.
The bioreactor according to any one of the preceding claims, further comprising one or more filter membranes.
An apparatus comprising:
- an upper tank for receiving therewith a liquid medium and a lower tank in fluid communication with said upper tank via a flow passage;
- a plurality of ports, each configured to facilitate fluid communication between the interior and the exterior of the apparatus;
- a filter membrane disposed such that liquid flowing from said upper tank to said lower tank mast pass therethrough;
- a stirring mechanism configured to mix the contents of the upper tank;
- an ultrasonic shaker disposed adjacent the filter;
- and
- a controller configured to direct operation of the stirring mechanism and ultrasonic shaker, and to facilitate passage of liquid from the upper tank to the lower tank.
A connection panel configured to facilitate functionally connecting a bioreactor to a control unit, said connection panel being separate from the bioreactor and from the control unit, the connection panel comprising a printed circuit board with a plurality of nodes, each of said nodes being configured for connection to the control unit and connection to an element associated with operation of the bioreactor for functionally connecting it to the control unit.
The kit according to any one of the preceding claims, wherein at least some of said elements are disposable.
Dependencies are inferred from the claim text.