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US 2024/0182850 A1Published application
Vitro Production of Medial Ganglionic Eminence Precursor Cells
AssigneeThe Regents of the University of California
InventorsCory R. Nicholas, John L. Rubenstein, Arnold R. Kriegstein, Arturo Alvarez-Buylla
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Abstract
Methods and systems for generating MGE precursor cells in vitro as well as compositions of enriched MGE precursor cells are provided. The methods and systems provide efficient production of MGE precursors. The methods and systems disclosed herein provide functional MGE precursors which differentiate into functional GABAergic interneurons.
Claims21
-17. (canceled)
A method comprising:
- a) providing a cell culture enriched in MGE precursor cells, wherein the MGE precursor cells are generated from primate pluripotent stem (pPS) cells;
- b) introducing a Notch pathway inhibitor to the cell culture enriched in MGE precursor cells and culturing the MGE precursor cells to produce a cell culture enriched in GABAergic neuronal precursors.
A composition comprising:
- GABAergic neuronal precursors and a Notch pathway inhibitor,
- wherein the GABAergic neuronal precursors are produced in vitro from MGE precursor cells, or
- MGE precursor cells and a Notch pathway inhibitor,
- wherein the MGE precursor cells are produced in vitro from primate pluripotent stem cells.
1 more element
Dependencies are inferred from the claim text.