Method and device for porating and loading cells, especially immunocompetent cells

A device for porating, in particular for sonoporation or laser porating, and for loading cells, in particular immunocompetent cells, in particular γδ T cells, comprising:

• a. an inlet opening and an outlet opening for a cell solution with cells to be porated and at least one substance to be taken up by the cells as a load and preferably microbubbles, wherein a poration arrangement extends between the inlet opening and the outlet opening, wherein the poration arrangement has a collecting unit (99) arranged at the inlet opening for collecting the cell solution and a reactor in flow connection with the collecting unit, and wherein inside the reactor the cell membranes of the cells may be porated and the load may be uptaken by the cells, and
• b. at least one poration unit (104) arranged on the reactor, by means of which energy can be applied to the cell solution in such a way that the cell membranes of at least some of the cells are porated,
• wherein
• c. the poration arrangement comprises a tubular incubation section (113) between the reactor and the outlet opening, within which the porated cells can be incubated with the substance to be loaded for the purpose of enriching loaded cells.