Stable multispecific molecule and use thereof
AssigneeDaiichi Sankyo Company, Limited
InventorsMakiko NAKAYAMA, Kensuke NAKAMURA, Shinji FURUZONO, Ryota MATSUMOTO
Life of this patent
Estimating the term…
Abstract
To provide a stable bispecific molecule. A Fab that specifically binds to CD3, the Fab comprising the following CDRH1 to 3 and CDRL1 to 3: CDRH1 consisting of the amino acid sequence represented by SEQ ID NO: 32; CDRH2 consisting of the amino acid sequence represented by SEQ ID NO: 33; CDRH3 consisting of the amino acid sequence represented by SEQ ID NO: 34; CDRL1 consisting of the amino acid sequence represented by SEQ ID NO: 35; CDRL2 consisting of the amino acid sequence represented by SEQ ID NO: 36; and CDRL3 consisting of the amino acid sequence represented by SEQ ID NO: 37.
Claims69
A Fab that specifically binds to CD3, the Fab comprising the following CDRH1 to 3 and CDRL1 to 3:
- CDRH1 consisting of the amino acid sequence represented by SEQ ID NO:
- 32;
- CDRH2 consisting of the amino acid sequence represented by SEQ ID NO:
- 33;
- CDRH3 consisting of the amino acid sequence represented by SEQ ID NO:
- 34;
- CDRL1 consisting of the amino acid sequence represented by SEQ ID NO:
- 35;
- CDRL2 consisting of the amino acid sequence represented by SEQ ID NO:
- 36;
- and
- CDRL3 consisting of the amino acid sequence represented by SEQ ID NO:
- 37.
A scFv that specifically binds to CD3 and has a heavy chain variable region and a light chain variable region connected via a disulfide bond, the scFv comprising the following CDRH1 to 3 and CDRL1 to 3:
- CDRH1 consisting of the amino acid sequence represented by SEQ ID NO:
- 32;
- CDRH2 consisting of the amino acid sequence represented by SEQ ID NO:
- 33;
- CDRH3 consisting of the amino acid sequence represented by SEQ ID NO:
- 34;
- CDRL1 consisting of the amino acid sequence represented by SEQ ID NO:
- 35;
- CDRL2 consisting of the amino acid sequence represented by SEQ ID NO:
- 36;
- and
- CDRL3 consisting of the amino acid sequence represented by SEQ ID NO:
- 37.
A Fab that specifically binds to CD3 and comprises the following CDRH1 to 3 and CDRL1 to 3:
- CDRH1 consisting of the amino acid sequence represented by SEQ ID NO:
- 40;
- CDRH2 consisting of the amino acid sequence represented by SEQ ID NO:
- 41;
- CDRH3 consisting of the amino acid sequence represented by SEQ ID NO:
- 42;
- CDRL1 consisting of the amino acid sequence represented by SEQ ID NO:
- 43;
- CDRL2 consisting of the amino acid sequence represented by SEQ ID NO:
- 44;
- and
- CDRL3 consisting of the amino acid sequence represented by SEQ ID NO:
- 45.
A scFv that specifically binds to CD3 and has a heavy chain variable region and a light chain variable region connected via a disulfide bond, the scFv comprising the following CDRH1 to 3 and CDRL1 to 3:
- CDRH1 consisting of the amino acid sequence represented by SEQ ID NO:
- 40;
- CDRH2 consisting of the amino acid sequence represented by SEQ ID NO:
- 41;
- CDRH3 consisting of the amino acid sequence represented by SEQ ID NO:
- 42;
- CDRL1 consisting of the amino acid sequence represented by SEQ ID NO:
- 43;
- CDRL2 consisting of the amino acid sequence represented by SEQ ID NO:
- 44;
- and
- CDRL3 consisting of the amino acid sequence represented by SEQ ID NO:
- 45.
Dependencies are inferred from the claim text.