Method for producing immune cell composition
AssigneesNeogentc Corp., The Asan Foundation, University of Ulsan Foundation for Industry Cooperation
InventorsHee Jin LEE, Kyu Jin LEE, Hye Seon PARK, Gyung Yub GONG, Chae Lyul LIM, Young Ae KIM, Ji ll CHOI
Life of this patent
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Abstract
A method for producing an immune cell composition is disclosed. The method includes steps of: (a) culturing a tumor sample in a first container, (b) obtaining a first immune cell population from the cultured product obtained from (a); (c) seeding a second composition and a second immune cell population in a second container; (d) expending the second immune cell population to produce a third immune cell population; and (e) obtaining the third immune cell population from the expanded product obtained from (d) and producing the immune cell composition.
Claims23
-48:
- (canceled)
A method for producing an immune cell composition, comprising:
- (a) culturing a tumor sample in a first container,
- wherein the tumor sample is cultured in a first composition in the first container, wherein the first composition comprises medium, serum, and 500 to 1500 IU/ml of IL-2,
- wherein the first container is a gas-permeable container or a container comprising gas supply system,
- wherein the culturing is performed for 13 to 15 days,
- wherein, during the culturing, a first additive composition is added to the first container once or twice or more, and the first additive composition comprises medium, serum, and IL-2;
- (b) obtaining a first immune cell population from the cultured product obtained from (a);
- (c) seeding a second composition and a second immune cell population in a second container,
- wherein the second immune cell population is derived from the first immune cell population,
- wherein the second composition comprises medium, serum, 1000 to 3000 IU/ml of IL-2, anti-CD3 antibody, and a feeder cell,
- wherein the second container is a gas-permeable container or a container comprising gas supply system;
- (d) expending the second immune cell population to produce a third immune cell population,
- wherein during the expansion, a second additive composition is added to the second container once or twice or more, and the second additive composition comprises medium, serum, and IL-2,
- wherein the expansion is performed for 13 to 15 days;
- and
- (e) obtaining the third immune cell population from the expanded product obtained from (d) and producing the immune cell composition.
Dependencies are inferred from the claim text.