Extracellular vesicles loaded with at least two different nucleic acids
AssigneeCarmine Therapeutics Pte. Ltd.
InventorsYumi Kawamura, Ronne Wee Yeh Yeo
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Abstract
Extracellular vesicles loaded with at least two different nucleic acids. An extracellular vesicle loaded with a cargo, wherein the cargo comprises at least two different nucleic acids and methods for preparing and using such extracellular vesicles.
Claims40
A population of extracellular vesicles loaded with a cargo, wherein the cargo comprises at least two different nucleic acid molecules and the population is prepared by contacting the extracellular vesicles with the cargo in the presence of a transfection reagent.
An extracellular vesicle loaded with a cargo, wherein the cargo comprises at least two different nucleic acid molecules.
The extracellular vesicle of any one of the preceding claims wherein the cargo comprises:
- a DNA and an RNA, at least two different mRNA molecules, a plasmid and a gRNA, an mRNA and a gRNA, a plasmid and an antisense oligonucleotide, a plasmid and a siRNA.
An extracellular vesicle comprising an mRNA or plasmid encoding an antigen-binding molecule or fragment thereof.
The extracellular vesicle of any one of the preceding claims wherein the extracellular vesicle is derived from a red blood cell.
A method for loading an extracellular vesicle with a cargo, the method comprising:
- a. providing a mixture, the mixture comprising cargo molecules to be loaded into an extracellular vesicle;
- and
- b. contacting with an extracellular vesicle under conditions sufficient for the extracellular vesicle to be loaded with the cargo molecules;
- wherein the mixture of cargo molecules comprises at least two different nucleic acid molecules.
Dependencies are inferred from the claim text.