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AssigneeR.J. Reynolds Tobacco Company
InventorsAnthony Richard Gerardi, Crystal Dawn Hege Byrd, Thaddeus Jude Jackson, Chelsea Allison Cooke, John-Paul Mua, Kyle Ford
Estimating the term…
The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and/or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and/or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 μg/g; and a heavy metal content of less than about 60 μg/g.
-27. (canceled)
A method for modifying the sensory characteristics of a protein-enriched material, the method comprising:
-39. (canceled)
A method for modifying the content or sensory characteristics of a plant-derived, protein-enriched material, the method comprising receiving a plant-derived, protein-enriched material comprising RuBisCO, F2 fraction proteins, or a combination thereof, wherein the plant-derived, protein-enriched material further comprises one or more undesirable characteristics or impurities selected from the group consisting of ash, nicotine, heavy metals, odor, taste, or a combination thereof;
The protein composition of claim 53, having an ash content of less than about 10% by weight.
The protein composition of claim 53, having a nicotine content of less than about 5 μg/g.
The protein composition of claim 53, having a heavy metal content of less than about 50 μg/g.
The protein composition of claim 53, having a heavy metal content of less than about 40 μg/g.
The protein composition of claim 53, wherein the combined concentration of cadmium, copper, lead, and nickel is less than about 40 μg/g.
The protein composition of claim 53, wherein the combined concentration of cadmium, copper, lead, and nickel is less than about 20 μg/g.
(canceled)
Dependencies are inferred from the claim text.